These different methods are well known in the art. Indeed, the presence of the expression product of a gene recognized by an antibody specific for said expression product can be detected by the presence of an antigen-antibody complex formed after contacting the strain of Listeria innocua or monocytogenes 4b or the microorganism associated with an antibody of the invention. The amplified nucleotide fragments may be used as reagents in hybridization reactions in order to highlight the presence l4, in a biological sample, of a sequence complementary to target nucleic acid to that of said amplified nucleotide fragments. In particular, can be performed in situ synthesis by photochemical addressing or by inkjet. Genes common to L.
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In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide Listeria monocytogenes 4b or innoczra 25 or a fragment thereof involved in the nucleotide metabolism, purine, pyrimidine or nucleosides. So while contamination by Listenia are very common, the number of cases described is low. This tool, consisting mintno the support containing the coding sequences used as a mixture of molecules labeled hybridization miinton reflecting the messenger RNAs expressed in the cell particularly labeled probes of the invention.
A protein chip described above can be used for the detection of gene products, to establish an expression profile of said genes, in addition to a DNA chip according to the invention.
Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in transport processes and protein binding. Unmarked sequences of polynucleotides of the invention can be used directly as a probe or primer. The present invention also relates to the genomic sequence and nucleotide sequences encoding polypeptides of Listeria inrzocua, such as cellular envelope polypeptides, secreted or specific, or involved in the metabolism and the replication process, as well as vectors including the said sequences and cells or animals transformed with these vectors.
The representative fragments according to the invention may also be probes or primers which can be used in methods of detection, identification, assay or amplification of nucleic sequences. The polynucleotide or vector of the invention may also be suspended in a buffer solution or be associated with liposomes.
The nucleotide sequence constituting the vaccine composition of the invention may be injected into the host after having been coupled to compounds which promote the penetration of this polynucleotide inside the cell or its transport to the cell nucleus.
Preferably, a transformed cell is cultured by a vector mintom the invention under conditions which allow expression of said polypeptide and said recombinant peptide mmpp recovered. PCR-like is meant all methods using aeuvre direct or indirect reproductions of nucleic acid sequences, or in which the labeling systems have been amplified, these techniques are of course known. One can fix the probes or primers according to the first invention on solid supports, in particular DNA microarrays, by different manufacturing processes.
By way of illustration, conditions of high stringency of the hybridization step for the purposes of defining the polynucleotide fragments described above are advantageously the following. These different elements are chosen and optimized by the skilled person depending on the host cell used.
It is understood that one calculates the percent identity between two polypeptides in the same way between two nucleic acid sequences.
CA2424952A1 – Listeria inocua, genome and applications – Google Patents
The reference sequences used were: The amplified nucleotide fragments may be used as reagents 3107 hybridization reactions in order to highlight the presence l4, in a biological sample, of a sequence complementary to target nucleic acid to that of said amplified nucleotide fragments. As regards the vaccine formulations, these may comprise appropriate immunity of the adjuvants which are known to those skilled in the art, such as aluminum hydroxide, a representative of the family of muramyl peptides as one of the peptide derivatives of N-acetyl-muramyl, a bacterial lysate, or alternatively incomplete Freund’s adjuvant.
It also provides a pernettant tool to identify genes whose expression follows a specific pattern. One such method of preparation is also an object of the invention.
Is intended to denote a DNA chip or high density filter, a support on which are fixed DNA sequences, each of which may be identified by its geographical location. The invention also includes host cells transformed with a vector according to the invention. Some Jinton strains should therefore have the ability to be transformed. Following the discovery of contamination, or typing of isolates is required to identify the mlnton of contamination.
Their modes of administration, mintln and galenic forms can mp determined according to criteria generally considered in establishing a suitable treatment for a patient such as age or body weight of the patient, the severity of the general condition, tolerance to the treatment and the side effects. Preferably, the probes or primers of the invention are immobilized on a support, covalently or noncovalently.
The differences between the genomic sequences of different strains or species can greatly affect the intensity of hybridization and, therefore, interfere with the interpretation of results. Such monoclonal or polyclonal antibodies, their fragments, or chimeric antibodies recognizing polypeptides according to the invention, are also subjects of the invention. CLIP strain is an epidemic strain. More information on Crossmatch Phrap and software are mml on the website http: October 2, under No.
Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a specific polypeptide of Listeria innocua or monocytogerzes 4b or a fragment thereof.
Thus, the percent identity between two polypeptides is determined after optimal alignment of the two sequences over a window of maximum homology.
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Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of this cell envelope or Listeria innocua or surface monocytogenes 4b or to a fragment thereof. These could be of the “chip” with DNA or another type.
Antibodies according to the invention are for example chimeric antibodies, humanized antibodies, Fab, or F ab ‘ 2.